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The Fresno County Coroner's Office identified him as 41-year-old Luciano Becerra Garcia of Fresno.
The crash happened just before 5 a.m. Friday in a northbound lane of Highway 41 near Shields Avenue.
The California Highway Patrol says Garcia was riding his bike against traffic when a 19-year-old driver hit him.
Garcia was thrown into the roadway, blocking two lanes of traffic.
He was taken to the hospital but died from his injuries.
The CHP says the driver stopped and cooperated with investigators.
It is unknown if alcohol and/or drugs were a factor for the bicyclist.
The investigation is ongoing.