Allan Cardoso pleaded not guilty to one count of murder and two counts of attempted murder.
The Fresno County Sheriff's Office says 17-year-old Gregory Sanchez was shot and killed near Highway 145 and Yuba Avenue in Five Points in January 2020.
Deputies say an 18-year-old was also hit when someone shot at their vehicle from another car.
A third person was not injured.
Investigators believe the shooting involved gangs.
Cardoso was arrested in February of this year.
His next court date is set for May 29th.